Dear Scientist,

I am currently writing my Maturaarbeit and have several questions regarding my topic. In my work, I am investigating the effects of rising ocean acidity on the rate of photosynthesis in algae. To measure this rate, I am planning to use a DCPIP solution, which changes color at a respective rate, measured by a Spectro Vis Plus device.

Ideally, I would like to predict the effects on the ocean system when the pH falls by 0.1, 0.2, etc. For this, I am considering using four different types of algae: one that forms the food basis for animals, one that is important for coral reef building, another that creates a different kind of habitat, and finally, an invasive species that causes native algae to die or is otherwise disruptive. However, I am unsure which specific algae species to use. Could you provide some guidance?

My second question arose after discussing with my supervisor (my biology teacher). She mentioned that she has no idea whether a changing pH has any effect on the DCPIP solution, meaning it might not be reliable for measurements in this context. I would like to know if a highly acidic pH affects DCPIP and, if so, what alternative methods or indicators I could use to measure the rate of photosynthesis.

I hope I have explained my Maturaarbeit in a way that is understandable and that you can assist me with my questions.

Thank you for your help.

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